Pump–probe microscopy

Pump–probe microscopy is an advanced imaging technique that allows researchers to study ultrafast dynamic processes in materials and biological systems at high spatial and temporal resolution. The technique typically involves two sequential laser pulses: the "pump" pulse and the "probe" pulse. 1. **Pump Pulse**: The first pulse, known as the pump, is used to excite the sample. This excitation can involve processes like electronic transitions, vibrational excitations, or changes in the structural configuration of the material.